A1120 wizard genomic dna purification kit components. Due care should be exercised with all promega products to prevent direct human contact. Promega real time pcr qpcr and rtqpcr systems provide a choice of probebased or dyebased qpcr methods. Product components and storage conditions product size cat. The sv total rna isolation system provides a fast and simple technique for preparing purified and intact total rna from tissues, cultured cells and white blood cells in as little as one hour, depending on the number of samples to be processed. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. A1120wizard genomic dna purification kitgben promega. Apparently in the manual isolating genomic dna from plant tissue, addition of cell lysis. Both probe and dyebased systems provide sensitive detection for reproducible and earlier quantification of low and highcopynumber targets over a broad dynamic range, together with resistance to a wide range of pcr inhibitors. For chemical emergency spill, leak, fire, exposure, or accident call chemtrec day or night within usa and canada.
Promega corporation 2800 woods hollow road madison, wi 53711 u. Like the wizard plus sv dna purification system cat. We encourage you to search our online catalog using the search feature above. Dna iq systemsmall sample casework protocol promega.
Gotaq g2 hot start green master mix is a premixed readytouse solution containing gotaq g2 hot start polymerase, dntps, mgcl 2 and reaction buffers at optimal concentrations for efficient amplification of dna templates by pcr. Luciferase assay system, 10pack e1501 from promega. Wizard sv genomic dna purification system protocol promega. Restriction enzyme activity in promega 10x buffers.
Wizard genomic dna purification kit protocol promega. Briefly centrifuge the pgem t or pgem t easy vector and control insert dna tubes to collect the contents at the bottom of the tubes. Promega ultra pure deoxynucleotide triphosphates dntps sets. If you have questions or need help with a product, feel free to contact us. Promega offers genomic dna isolation systems based on sample lysis by detergents and purification by various methods. Promega ultra pure deoxynucleotide triphosphates dntps sets set of datp, dctp, dgtp and dttp. In many cases, good activity is also obtained using one. The wizard genomic dna purification kit is designed for isolation of dna from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, grampositive and gramnegative bacteria. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 phone 6082744330 fax 6082772516. Each promega product is shipped with documentation stating specifications and other technical information. Promega products contain chemicals which may be harmful if misused. The wizard sv gel and pcr cleanup system extracts dna fragments of 100bp to 10kb from standard or lowmelt agarose gels in either tris acetate tae or tris borate tbe and purifies pcr products directly from an amplification reaction. The assay sequentially measures two protease activities. Life science supply center 1120 nat sci biology dispensary.
The luciferase assay system is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Promega wizard plus sv minipreps dna purification system. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Pianissimo playing the last grave on the right, we hear only the stem to a harmonic shrill. View and download generalmusic promega 2 owners manual online. Promega does not encourage or support the unauthorized or unlicensed use of the pcr process. Restriction enzyme activity in promega 10x buffers, reaction temperature and heat inactivation restriction enzyme activity in promega 10x buffers, reaction temperature and heat inactivation. Email promega technical services if you have questions on use of this system. Certificate of analysis promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 6082772516 internet.
Protocol for ligations using the pgem t and pgem t easy vectors and the 2x rapid ligation buffer 3. Certificate of analysis gotaq green master mix cat. Gotaq g2 hot start green master mix protocol promega. Promega wizard plus kit 50 preps visit or call prr6021 bam hi 2500u visit or call pr a1120 promega wizard genomic. Description the pgl4 luciferase reporter vectorsae are the next generation of reporter gene vectors optimized for expression in mammalian cells.
Instructions for use of products a9281, a9282, a9285. To perform a spectral calibration with the promega 4dye chemistry for the first time, create a new dye set. A1120wizard genomic dna purification kitusen promega. Instructions for use of products a1120, a1125, a1620. Adamia therapeutic repair lotion with macadamia nut oil and promega 7, 16 oz bottle fragrance free, paraben free, non gmo. Wizard genomic dna purification kit 100 isolations a1120 each system contains sufficient reagents for 100 isolations of genomic dna from 300l of whole blood samples. Linear results are seen over at least 8 orders of magnitude of enzyme concentration, and the patented technology incorporated into this assay system allows measurement of less than 1020 moles of luciferase under optimal conditions. The 10x reaction buffer supplied with each restriction enzyme is optimized to give 100% activity. I am trying to extract dna from some fungi species mold using wizard genomic dna purification kit.
Because purchase of this product does not include a license to perform any patented application, users of. Revised 819 tb296 technical bulletin dna iq systemsmall sample casework protocol instructions for use of products dc6700 and dc6701. Description the celltiter glo luminescent cell viability assayad is a homogeneous method to determine the number of viable. Regardless of the system chosen, promega genomic dna purification kits provide the required yields of highquality dna with minimal contaminants. The multitoxglo multiplex cytotoxicity assay is a sequentialreagentaddition fluorescent and luminescent assay that measures the relative number of live and dead cells in cell populations. A1120 wizard genomic dna purification kit, 100 isolations 243,000 195,000 20% a1125 wizard genomic dna purification kit, 500 isolations 606,000 485,000 a1620 wizard genomic dna purification kit, 100 isolations. The only small diffrence is that with the truth was even more of reserves to attack lgrement saturate the stamp the kind of vloc promega are anchored te 127, fender is up 140, but its really for the quibble. Pureyield plasmid midiprep system protocol promega. Promega products are warranted to meet or exceed the stated specifications. The miniprep procedure can be completed in 45 minutes or less. Promega wizard plus sv minipreps dna purification system a silica membranebased system for simple, rapid isolation of plasmid dna from 110ml e. Announcing the 2020 promega catalog we are proud to offer topoftheline products and services to our customers.